![]() Multiplex assays are supported in the TaqMan® design mode. Read about how TaqMan® probes work! Multiplex Real Time PCR Assay Designīeacon Designer™ uses innovative proprietary algorithms to design optimal primer-probe sets for single tube multiplex assays. You can also specify their placement and the LNA free region on each end of the probe to design LNA™ spiked TaqMan® probes. You can specify the number of LNA™ bases that you wish to have in the probe. LNA™ substituted TaqMan® probes are shorter probes and have LNA™ bases that impart higher stability. Beacon Designer™ can design LNA™ probes.You can even import the primers designed in the SYBR® Green primer design mode and design a compatible probe.ĭisplays a graphical view of TaqMan® probe secondary structures. Similarly, a pre-designed TaqMan® probe can be evaluated and compatible primers be designed. Pre-designed primer sets can be evaluated and a compatible TaqMan® probe be designed. Screens SYBR® Green primers for thermodynamic properties and secondary structures.Īvoids homologies with all other primers to reduce primer dimers in a multiplex reaction.ĭesigns optimal TaqMan® probes free of dimers, repeats and runs to ensure signal fidelity.ĭesigns wild and mutant TaqMan® probes for SNP identification for SNP genotyping assays Presents the design results in a ranked order. It then proceeds to design optimal dual labeled probes for use with well proven SYBR® Green primers.įor SNP genotyping studies, designs SYBR® Green primers such that the SNP site at or towards t he 3' end position.Ĭalculates SYBR® Green primer Tm using nearest neighbor thermodynamic algorithm. This functionality helps visualize where the designed oligos are located with respect to both the regions.Įvaluates pre-designed primers and designs a compatible primer a given sense or anti-sense primer. This helps verify the SYBR® Green primer design and visualize primer and amplicon specificity.īeacon Designer™ identifies template structures and then automatically avoids them while designing SYBR® Green primers.ĭisplays the regions of homology and secondary structures for the sequence graphically in the Sequence View pane. The SYBR® Green primers designed can be BLAST searched against genomic databases available at NCBI. Beacon Designer™ Features SYBR® Green Primer Designĭesigns primers optimized for SYBR® Green PCR assays.īeacon Designer™ designs highly specific SYBR® Green primers by BLAST searching sequences, automatically interpreting the results and then designing SYBR® Green primers by avoiding regions of significant homology.
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